Mise-en-scène is used throughout the opening to evoke different emotions in the viewer, and to introduce several of the film's main themes, such as betrayal, abandonment and the loss of innocence.

Most notably, images such as a close-up on the dead inventor introduce the viewer to Edward's grief at the loss of his creator and the first human he felt an emotional attachment to. This helps the viewer to have an emotional attachment to Edward, which makes the rest of the film more compelling for them. The image of the dead inventor also relates to the film's themes of loss and abandonment. Edward feels abandoned by the inventor when he dies, and this is his first experience with loss. This is important because it marks the beginning of Edward's loss of innocence and also shows how effected he was by this, furthering the viewer's understanding of his character.
The recurring images of snow introduce a motif used throughout the film. At the very beginning, Kim's granddaughter asks the question, "where does snow come from?" In a way, the rest of the film is spent exploring this question, eventually revealing that in the case of the town, the snow is created by Edward carving ice statues, his way of expressing his love for Kim. The use of snow in the opening introduces this theme and shows how Edward has difficulty expressing his emotions conventionally, both because of his physical disability and because of his differences from 'normal' society.

Frequently, images of disused machinery and statues covered in cobwebs are used to convey the film's recurring theme of abandonment. Much like the house he inhabits, Edward is abandoned by the townsfolk for being outside the norm, and left to gather dust and be forgotten. The use of disused machinery represents this visually, helping the viewer to clearly understand one of the film's main themes, and also the film's main character.

This image, of a ghoulish statue covered in cobwebs, is shown along with the name of the actor who plays the titular character. The statue, like Edward, has been abandoned and left alone. This visual display of isolation helps the viewer to become emotionally invested in the film in the opening seconds, and to begin to understand the main character before he is even introduced.
Burton uses this technique in the openings of some of his other films, such as The Nightmare Before Christmas, in which different parts of Halloween Town are explored during the opening to give the viewer a visual sense of the world Jack Skellington inhabits. This helps the viewer to understand Jack's character and why he is so fascinated by the concept of Christmas Town, which becomes the main plot of the film. This use of Mise-en-scène in the opening is used to great effect by Burton in both films to give greater understanding to the viewer about the nature of the film, important themes and emotions, and an introduction to main characters and what drives them.